FastQCFastQC Report
Mon 6 Jun 2016
ERR1378994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198018
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT64460.5380553547609468Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGACGATCTCGTATGCCGTC36320.3031673981526154Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGACGATCTCGTATGCC15880.13255226549183735Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.038.7824243
ATGCCGT4800.037.97445744
TGCCGTC5150.035.39366545
GATCGGA8050.034.9358526
TCGGAAG8400.034.5515528
ATCGGAA8350.033.68066827
AGATCGG8400.033.48018625
GACCGTA5950.030.62846226
CGTAGAC5950.030.62846229
CCGTAGA6000.030.37322628
ACCGTAG6000.030.37322627
AATGCCG15650.030.0461718
TAGACGA6150.029.63241431
GTAGACG6150.029.63241430
ATGCCGA15800.029.61852319
CGTATGC6250.029.16316441
AGACCGT6200.029.03056125
TGCCGAG16050.029.01699420
GAGATCG9900.028.4074324
GAATGCC16650.028.24159417
Produced by FastQC (version 0.11.5)