FastQCFastQC Report
Mon 6 Jun 2016
ERR1378993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209914
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT59660.49309289751172397Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGACAATCTCGTATGCCGTC33000.27274665802693415Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8050.036.05381826
ATCGGAA8150.035.61143527
TCGGAAG8400.035.355128
TATGCCG3450.033.91528743
AGATCGG8600.033.74804725
AATGCCG12600.033.74804718
ATGCCGA12600.033.39092319
TGCCGAG13050.032.23951320
CCGAGAT9300.031.2078722
CGGAAGA9550.031.09767729
CGAGATC9550.030.62650123
TGCCGTC3750.030.60202445
CGCAGAC4050.030.55378529
ATGCCGT3800.030.19936644
CCGCAGA3900.029.99826228
GCCGAGA13800.029.8352321
CGTATGC4000.029.8132441
GAATGCC14450.029.42736217
AGACCGC4000.029.2483125
GAGATCG10200.028.67481224
Produced by FastQC (version 0.11.5)