FastQCFastQC Report
Mon 6 Jun 2016
ERR1378992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663383
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT42590.6420122312449973Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTAGATCTCGTATGCCGTC23600.3557522577455256Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTAGATCTCGTATGCC7680.11577022624939137Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3600.036.2540743
TCGGAAG5050.035.64219328
ATCGGAA5050.035.64219327
GATCGGA5150.034.51323326
ATGCCGT3850.034.48438644
CGGTGTA3950.033.606329
TGCCGTC4100.033.47936645
AATGCCG9650.033.10753318
AGATCGG5450.032.61342225
GACCGGT4100.032.376826
ATGCCGA10000.031.9487719
TCGTATG4300.031.39413840
TGCCGAG10200.031.32232320
TCTCGTA4100.031.27928238
CTCGTAT4250.031.2340939
CCGAGAT5700.031.18300822
CGGAAGA6000.031.12380229
ATCTCGT4150.030.90242637
CCGGTGT4300.030.87090328
GAATGCC10500.030.85595517
Produced by FastQC (version 0.11.5)