FastQCFastQC Report
Mon 6 Jun 2016
ERR1378991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011978
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT59280.5857834854117382Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCCGTC34960.34546205549923026Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCC16570.1637387374033823Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4600.034.74355744
ATCGGAA7450.034.42597627
TATGCCG4550.034.1359143
GATCGGA7550.033.97000526
TCGGAAG7850.033.24497628
AGATCGG8100.031.94114925
AATGCCG13750.030.43322218
TGCCGTC5100.030.01321845
ATGCCGA14100.029.67778819
TGCCGAG14400.029.05949820
CCGAGAT9250.028.45652222
CGAGATC9300.028.06161923
GAATGCC15300.027.35011717
GAGATCG9550.027.32702324
CGGAAGA9850.027.17993529
CGACGTA5800.026.37658729
CTCGTAT6050.025.66484639
CGTATGC6000.025.50745241
CCGACGT6000.025.49736828
GCCGAGA16250.025.47429521
Produced by FastQC (version 0.11.5)