FastQCFastQC Report
Mon 6 Jun 2016
ERR1378990.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378990.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254436
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT52490.4184350576673501Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTAGATCTCGTATGCCGTC23240.18526254029699402Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3100.035.56801643
ATGCCGT3650.031.44144844
ATCGGAA6550.030.91414327
TCGGAAG6750.030.66479328
GATCGGA6650.030.44926626
TGCCGTC3900.028.84899145
AGATCGG7100.028.51938425
AATGCCG11800.027.07461718
ATGCCGA12150.026.47986219
TGCCGAG12200.026.18692420
CTCGTAT4300.026.16223539
CGCAGTA4300.026.16119429
CCGAGAT7900.025.63134622
AGACCGC4450.025.27935425
CGGAAGA8300.024.93823429
CGAGATC8400.024.37350823
GCCGAGA13100.024.04433321
TCGTATG4700.023.93566140
CCGCAGT4700.023.9347128
TCTCGTA4750.023.68370638
Produced by FastQC (version 0.11.5)