FastQCFastQC Report
Mon 6 Jun 2016
ERR1378988.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378988.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099647
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT87280.7937092539696831Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCCGTC46910.42659144252655623Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGACATCTCGTATGCC21970.19979138759983883Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC12270.11158126198680123Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5800.039.57008443
ATGCCGT6250.037.08104744
TGCCGTC6350.036.14275445
GATCGGA11750.034.2744526
ATCGGAA11700.034.22862227
TCGGAAG12100.033.2830428
AGATCGG12150.033.14607225
CCGAGAT13200.030.85034222
CGAGATC13700.029.56019223
GAGATCG13900.029.13486524
AATGCCG22700.028.14801418
TGCCGAG23050.027.81821320
ATGCCGA23000.027.78086919
GAATGCC23800.026.9415917
GCCGAGA23750.026.80884721
CGGAAGA15250.026.55571429
GGAATGC24650.026.0125716
CGAGTGA9200.025.43318729
TCTCGTA9000.025.24956738
TCGTATG9000.025.24956740
Produced by FastQC (version 0.11.5)