FastQCFastQC Report
Mon 6 Jun 2016
ERR1378987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612531
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT75171.2272031946138235Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGACATCTCGTATGCCGTC44410.7250245293707585Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGACATCTCGTATGCC17340.2830877131116629Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10850.17713389199893556Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5250.039.0047443
ATGCCGT5450.036.7475944
GATCGGA9200.035.70211426
ATCGGAA9150.035.6513427
TCGGAAG9800.035.3527828
AGATCGG9300.035.0763225
TGCCGTC5800.034.91803745
CCGAGAT9950.033.23710622
CGAGATC10100.032.29799323
GAGATCG10100.032.29799324
GGTCGGA651.4029865E-831.15000526
AATGCCG17800.030.96528218
TGCCGAG17950.030.58118620
ATGCCGA18000.030.49623719
CTACGCC300.00514850829.99630228
GCCGAGA17950.029.45319221
GAATGCC18850.029.12345517
TCTCGTA7050.028.40538638
CGGAAGA12200.028.21373229
ACCGGAT7050.028.08164227
Produced by FastQC (version 0.11.5)