FastQCFastQC Report
Mon 6 Jun 2016
ERR1378986.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378986.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914637
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT75820.8289627469695628Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCCGTC45480.4972464485910804Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCC13960.1526288571312991Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11560.12638893899984366Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4650.041.6231243
TGCCGTC5000.038.709545
ATGCCGT5050.038.32623744
GATCGGA9600.034.9189926
ATCGGAA9750.033.68952627
TCGGAAG10100.033.63583428
AGATCGG10450.032.2939825
CCGAGAT10550.031.98787922
CGCATGA6250.030.5974729
AATGCCG17700.030.50595318
TGCCGAG17900.030.41648120
CGAGATC11100.030.40289523
CGTATGC6400.030.24014341
TCGTATG6400.030.24014340
GAGATCG11250.030.19750624
ATGCCGA18050.029.91442519
TCTCGTA6500.029.4286938
CCGCATG6600.029.31575828
CTCGTAT6600.028.98280139
GAATGCC18800.028.72103117
Produced by FastQC (version 0.11.5)