FastQCFastQC Report
Mon 6 Jun 2016
ERR1378985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981696
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT67070.6832053914857553Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGAGATCTCGTATGCCGTC43770.44586104048503816Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGAGATCTCGTATGCC11250.11459759436729905Illumina Paired End PCR Primer 2 (97% over 36bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10120.1030869026664059Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.036.7376943
ATGCCGT5000.035.552944
TGCCGTC5150.034.51737645
GATCGGA9500.033.86773326
ATCGGAA9600.033.74931327
TCGGAAG10050.033.3575328
AGATCGG9800.032.83096325
CCGAGAT10800.029.99939222
CGATTGG300.0051474129.9993884
TGGTACG300.0051474129.99938824
GACCGAT6100.029.87644226
AATGCCG17400.029.74077218
ACCGATC6150.029.63354327
ATGCCGA17600.029.27497319
CGGAAGA11500.029.1515829
TCGAGAT6300.028.9279832
CGATCGA6300.028.9279829
GAGATCG11200.028.9279824
TGCCGAG17800.028.81963720
CGTATGC6250.028.8008841
Produced by FastQC (version 0.11.5)