FastQCFastQC Report
Mon 6 Jun 2016
ERR1378983.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378983.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003208
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT51800.5163435698279918Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATGCAGATCTCGTATGCCGTC26010.25926826739818665Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGCAGATCTCGTATGCC14980.14932097830160843Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGA250.002107473835.99828725
ATCGGAA7050.032.55164327
GATCGGA7100.032.32240326
TATGCCG3000.032.2532943
TCGGAAG7200.032.18596628
AGATCGG7500.030.8985325
ATGCCGT3150.030.71741744
TGCCGTC3350.029.5552545
AATGCCG12250.026.99871318
CGAGATC8700.026.89527123
ATGCCGA12400.026.67211719
CGGAAGA8750.026.48445329
CCGAGAT8750.026.22732222
TGCCGAG12800.025.83861520
GAGATCG9000.025.74877224
GAATGCC13350.024.6055717
CGATGCA4050.023.33222229
GGAATGC14500.023.11958916
CCGATGC4100.023.04768228
TCGTATG4200.023.03576740
Produced by FastQC (version 0.11.5)