FastQCFastQC Report
Mon 6 Jun 2016
ERR1378982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687832
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT45010.6543749055001802Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCAGATCTCGTATGCCGTC23160.33671012689145025Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCAGATCTCGTATGCC12380.17998581048860768Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3300.035.4667543
GATCGGA5850.034.61219826
AGATCGG5900.034.31887425
ATCGGAA5900.033.93755327
ATGCCGT3400.033.7616244
TCGGAAG6200.033.3840228
GAGATCG6400.031.63771224
TGCCGTC3700.031.02418945
CCGAGAT6700.030.22109822
CGAGATC6700.030.22109823
AATGCCG11450.028.49082818
ATGCCGA11550.028.24415419
CGGAAGA7350.027.85457829
TGCCGAG11750.027.76340120
GCCGAGA12050.026.69878821
GAATGCC12350.026.41457217
GGAATGC12600.025.89047216
CCGTAGC4650.025.64280128
GACCGTA4750.024.6293126
CGTAGCA4750.024.6293129
Produced by FastQC (version 0.11.5)