FastQCFastQC Report
Mon 6 Jun 2016
ERR1378981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989284
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT37760.38169019209852784Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGCATATCTCGTATGCCGTC21370.2160148147549137Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGCATATCTCGTATGCC10170.10280162218331641Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG5000.035.09755325
ATGCCGT3400.035.0781844
TCGGAAG5200.035.04563528
TATGCCG3500.034.07594743
GATCGGA5250.033.42624326
ATCGGAA5250.033.42624327
AATGCCG9700.030.61642518
TGCCGTC4000.030.37902545
ATGCCGA10250.029.19308919
TGCCGAG10300.028.83294120
GAATGCC10550.028.36295317
AGACCGG4550.026.20696825
CGGAGCA4550.026.20696829
CGAGATC6800.025.80702623
ACCGGAG4650.025.64337527
CCGGAGC4650.025.64337528
CGGAAGA7150.025.48773629
CCGAGAT6900.025.43301222
TCGTATG4800.025.3107440
GCCGAGA11700.025.19055421
Produced by FastQC (version 0.11.5)