FastQCFastQC Report
Mon 6 Jun 2016
ERR1378978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224784
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT58250.4755940639329057Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCCGTC27020.2206103280251865Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCC13240.10810069367333341Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG7900.037.87644625
TCGGAAG8200.037.0394628
GATCGGA8500.035.20281626
ATCGGAA8500.035.20281627
TATGCCG3550.033.59959443
ATGCCGT3950.031.90637444
AATGCCG13500.031.33060618
ATGCCGA13750.030.76095819
TGCCGAG13950.030.31993920
CCGAGAT10050.029.77352522
CGGAAGA10400.029.20418729
CGAGATC10350.029.127923
GAGATCG10350.029.127924
TGCCGTC4200.028.3996645
GAATGCC15500.027.2890617
GCCGAGA15350.027.11490221
GGAATGC16850.025.50327316
GACCGTC5050.023.61180526
GAAGAGC13200.023.17979831
TCTCGTA5150.023.15521438
Produced by FastQC (version 0.11.5)