FastQCFastQC Report
Mon 6 Jun 2016
ERR1378977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321010
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT68490.5184669306061271Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCCGTC37710.2854633954322829Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.038.37619843
ATGCCGT5250.034.7213244
TCGGAAG9900.033.40574628
GATCGGA9950.032.55955526
AGATCGG10000.032.39675525
ATCGGAA10050.032.23557727
TGCCGTC5800.031.04077545
TCGTATG5950.031.00999540
TGCCGAG16750.030.89243120
ATGCCGA16800.030.53381519
CGTATGC5900.030.51119641
AATGCCG16850.030.44320918
CTCGTAT5950.030.25365339
CGGAAGA11000.029.65612429
TCTCGTA6100.029.14083938
AGACCGT6350.028.69791425
CCGAGAT11600.028.31613222
CCGTGTC6300.028.21146228
CGAGATC11700.028.07411223
GAGATCG11750.027.57170924
Produced by FastQC (version 0.11.5)