FastQCFastQC Report
Mon 6 Jun 2016
ERR1378974.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378974.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239787
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT68620.5534821707277137Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTGTATCTCGTATGCCGTC38990.3144895050520775Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTGTATCTCGTATGCC23740.19148450499964914Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.041.6567443
ATGCCGT5250.038.15000544
TGCCGTC5300.037.790145
GATCGGA9200.034.23587826
ATCGGAA9250.033.807627
AGATCGG9400.033.50745425
TCGGAAG10000.032.62190228
CGAGATC10550.030.28148323
CCGAGAT10800.029.58052322
AATGCCG18100.029.33422918
GAGATCG10900.029.1027424
ATGCCGA18350.028.93458219
TGCCGAG18650.028.46914520
GAATGCC19400.027.36853417
GCCGAGA19550.026.46807321
CGTACTG7550.026.22267529
CTCGTAT7650.025.8819839
CGTATGC7600.025.75828741
GGAATGC21000.025.71184316
CGGAAGA12950.025.71184229
Produced by FastQC (version 0.11.5)