FastQCFastQC Report
Mon 6 Jun 2016
ERR1378971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049455
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT54830.5224616586704528Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCCGTC26960.25689524562749233Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCC12620.12025289316835881Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3200.036.56865743
GATCGGA7450.035.6350926
ATCGGAA7650.034.4093627
ATGCCGT3550.034.23111344
AGATCGG7900.033.60524725
TCGGAAG8200.033.198928
TGCCGTC3750.031.80535345
AATGCCG12750.030.88019418
CCGAGAT8750.030.59786222
TGCCGAG12900.030.52112420
ATGCCGA13150.029.94087419
CGGAAGA9150.029.7520229
GAGATCG9150.029.01436624
CGAGATC9200.028.85667823
GCCGAGA13850.027.615421
TCTCGTA4300.027.20869638
TCGTATG4300.027.20869640
GAATGCC14700.026.93689317
GACCGTA4300.026.68418126
CTCGTAT4450.026.2915539
Produced by FastQC (version 0.11.5)