FastQCFastQC Report
Mon 6 Jun 2016
ERR1378968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230451
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50010.4064363391959534Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCCGTC30780.2501521799730343Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.041.14375343
ATGCCGT4000.036.56329344
ATCGGAA6850.034.8154427
TGCCGTC4250.034.4125145
TCGGAAG7050.033.8277728
AGATCGG7350.032.7531525
GATCGGA7300.032.66928526
CGTATGC4450.032.3589441
AATGCCG12450.030.72106218
TCTCGTA4700.030.63771438
ACCGTGA4800.030.46693427
ATGCCGA12600.030.35533319
CTCGTAT4750.030.31521439
TGCCGAG12800.030.056820
CGGAAGA8250.029.18007929
TATCTCG5100.028.67592836
TCGTATG5200.028.5571540
CCGAGAT8600.028.51574322
GACCGTG5050.028.51315126
ATCTCGT5100.028.2347637
Produced by FastQC (version 0.11.5)