FastQCFastQC Report
Mon 6 Jun 2016
ERR1378967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136658
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT56340.4956636033002011Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCCGTC30030.2641955627814171Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA8800.037.07219727
GATCGGA8850.036.8627526
AGATCGG8900.036.65565525
TCGGAAG9250.036.48484428
TATGCCG3850.036.2337343
CGGAAGA9750.034.38306829
AATGCCG14150.033.54973218
TGCCGAG14250.033.31429320
ATGCCGA14400.032.96726619
ATGCCGT4250.032.82349844
TGCCGTC4350.032.58617445
CCGAGAT10200.031.98385422
CGAGATC10350.031.73770323
GAGATCG10350.031.5203224
GAATGCC16000.029.95177817
CTCGTAT4700.029.67951639
GCCGAGA15650.029.6152821
TCTCGTA4800.029.06119338
TCGTATG4800.029.06119340
GACCGTC4950.028.63507526
Produced by FastQC (version 0.11.5)