FastQCFastQC Report
Mon 6 Jun 2016
ERR1378966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075402
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT86970.8087208318377686Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCCGTC44880.41733230922017994Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTACTATCTCGTATGCC17310.16096306311500258Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC12030.1118651443832167Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6050.039.79318243
ATGCCGT6450.038.0230644
TGCCGTC6800.036.0659945
ATCGGAA11400.034.73504627
TCGGAAG11550.034.67353428
GATCGGA11500.034.62864726
AGATCGG11550.034.4787425
CCGAGAT12750.031.76306522
CGTATGC7600.031.67746741
CGTGTAC7650.031.46896429
TATCTCG7700.031.26607336
TCTCGTA7700.031.26607338
AATGCCG20800.031.26040518
GAGATCG12750.031.2336824
CTCGTAT7850.030.95525239
GACCGTG7850.030.95381426
TCGTATG7800.030.86522340
ATGCCGA21050.030.78225719
CGAGATC13200.030.68023523
ATCTCGT7850.030.6686337