FastQCFastQC Report
Mon 6 Jun 2016
ERR1378963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556146
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT56891.0229328269914735Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCCGTC25580.4599511639030039Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCC19000.3416369082938653Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC6840.12298928698579151Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8250.035.4504426
ATCGGAA8050.035.2133127
TATGCCG3400.034.4201643
TCGGAAG8200.034.29480728
AGATCGG8500.033.87842625
CCGAGAT8900.032.86136222
ATGCCGT3700.032.23758744
CGAGATC9300.031.68987523
GAGATCG9300.031.44796824
TGCCGTC4000.029.81976945
AATGCCG14450.028.4940718
ATGCCGA14600.028.20132319
CCGTAAC406.1565655E-428.1293284
TGCCGAG14650.028.10254320
GAATGCC14900.027.93551617
GCCGAGA15250.027.14439421
GGAATGC15450.026.64979416
CGGAAGA10650.026.4053929
GAAGAGC11300.025.08558831
CGTAACA450.001224658925.0038495
Produced by FastQC (version 0.11.5)