FastQCFastQC Report
Mon 6 Jun 2016
ERR1378961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545889
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT63251.158660460276723Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCCGTC38840.7114999569509552Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCC27200.49826979477512834Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7740.14178706660145196Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4550.042.53232643
TGCCGTC4950.040.0082345
ATGCCGT4950.040.0045644
GATCGGA8450.036.2071926
AGATCGG8550.035.7837225
ATCGGAA8550.035.52060327
CCGAGAT8650.035.1099622
CGAGATC8650.035.1099623
TCGGAAG8950.034.68715728
GAGATCG9450.032.37574424
ATGCCGA17150.029.64537819
AATGCCG17200.029.559218
TGCCGAG17550.028.96969820
GAATGCC17900.028.27757517
GCCGAGA17700.028.21580121
GGAATGC18850.027.09113516
CGGAAGA11750.026.42128429
AGGAATG20050.025.46971915
CTCGTAT7950.024.62093539
CCGTACT8000.024.46481528
Produced by FastQC (version 0.11.5)