FastQCFastQC Report
Mon 6 Jun 2016
ERR1378960.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378960.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082538
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT67910.6273220893862387Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCCGTC29810.275371395738533Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCC18300.16904718356307122Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.035.7794443
GATCGGA8900.035.6430726
ATCGGAA9100.034.6124727
AGATCGG9250.034.29441525
TCGGAAG9450.033.8066828
ATGCCGT4300.032.97452544
TGCCGTC4400.032.22510545
CCGAGAT10200.031.54146422
CGAGATC10200.031.32089423
TGCCGAG14900.031.25575820
AATGCCG15100.030.9907718
ATGCCGA15100.030.84177619
GAGATCG10800.029.37252824
CGGAAGA10900.029.30945829
GCCGAGA16300.028.15713921
GAATGCC16950.027.87504817
CTCGTAT5100.027.35316739
TATCTCG5300.026.74550236
AGACCGC5250.026.56919325
TCGTATG5300.026.3209740
Produced by FastQC (version 0.11.5)