FastQCFastQC Report
Mon 6 Jun 2016
ERR1378959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831943
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT49720.5976370977338592Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTACATATCTCGTATGCCGTC24420.2935297249931786Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTACATATCTCGTATGCC13400.16106872706423397Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2550.037.9427543
AGATCGG7700.037.3996525
GATCGGA7750.037.1583626
ATCGGAA7750.036.8680627
TCGGAAG7850.036.39840328
TGCCGTC3050.031.72453145
ATGCCGT3100.031.2109744
GAGATCG9250.031.13268324
CGGAAGA9300.030.965329
AATGCCG12600.030.89042318
TGCCGAG12800.030.58352720
ATGCCGA12900.030.52085119
CCGAGAT9550.030.39027822
CGTTAAC300.00514692229.9994439
CGAGATC9600.029.99763523
GAATGCC14100.027.7637717
GCCGAGA14100.027.60420821
GGAATGC14650.026.56787116
CTCGTAT3800.025.4600539
ACCGCTA3950.025.06131627
Produced by FastQC (version 0.11.5)