FastQCFastQC Report
Mon 6 Jun 2016
ERR1378958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808453
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT57360.7095032116894859Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCCGTC26150.3234572696248267Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCC14270.17650995172261097Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3050.039.8390843
TGCCGTC3350.037.61478445
ATGCCGT3400.036.3998144
ATCGGAA8100.033.88725727
GATCGGA8300.033.07069826
AGATCGG8400.032.40915725
TCGGAAG8700.032.3260328
CCGAGAT8800.031.70301822
CGAGATC9400.029.91877623
AATGCCG14200.028.99508518
TGCCGAG14350.028.84878520
GAGATCG9600.028.82673824
ATGCCGA14450.028.49343919
CGGAAGA10150.027.70802929
GCCGAGA14800.027.05950721
GAATGCC15450.026.64920417
CGTATGC4600.026.41177441
TCGTATG4800.025.7800140
GGAATGC16150.025.77275516
GACCGTC4750.025.57771726
Produced by FastQC (version 0.11.5)