FastQCFastQC Report
Mon 6 Jun 2016
ERR1378957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311746
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41650.31751573856524057Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGTAATCTCGTATGCCGTC23010.17541505748826372Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5150.036.6972926
ATCGGAA5150.036.6972927
AGATCGG5400.035.41498625
TCGGAAG5400.034.99700528
TATGCCG3250.034.62430243
ATGCCGT3500.033.43718344
AATGCCG9450.031.90203318
CCGATAG3700.031.01085928
ATGCCGA9900.030.67919219
GACCGAT3750.030.59854726
TGCCGAG10100.030.29443720
CGATAGT3800.030.19478429
TGCCGTC3750.030.00772745
CGGAAGA6500.029.07443629
ACCGATA4050.028.3319927
CTCGTAT4100.027.44086339
GCCGAGA11450.026.72362321
AGACCGA4300.026.6847825
TCTCGTA4250.026.47235938
GAATGCC11600.026.18310417
Produced by FastQC (version 0.11.5)