FastQCFastQC Report
Mon 6 Jun 2016
ERR1378953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110590
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTCAAATCTCGTATGCC89640.8071385479790022Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC39880.3590884124654463Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCA13150.037.29966732
CCGAGTC13150.037.29966731
TCTCGTA12550.037.29012741
ATCTCGT12650.037.1732140
CTCGTAT12750.037.0581242
CGTATGC13050.036.3786244
TCGTATG13100.036.2397743
GAGACCG13550.036.03252427
GACCGAG13600.035.9000529
AGACCGA13600.035.9000528
ACCGAGT13700.035.8022430
AATCTCG13500.034.99933639
GTATGCC13400.034.9247145
CGAGACC14600.033.90346526
GAGTCAA15300.032.20527333
CGTTTCG300.005141999630.0061861
CGGAAGA17550.028.9803311
CCGAGAC17200.028.516925
GTCAAAT17150.028.0752735
AGTCAAA17500.028.0280434
Produced by FastQC (version 0.11.5)