FastQCFastQC Report
Mon 6 Jun 2016
ERR1378944.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378944.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795410
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGGCATCTCGTATGCC82601.0384581536566047Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC39010.49043889314944494Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT9210.11578934134597252Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG10200.035.9553332
GACCGCA10200.035.9553329
CCGCATG10250.035.77993431
AGACCGC10350.035.43423528
TCTCGTA9850.035.4055541
ATCTCGT9800.035.356640
GTATGCC9900.035.2267345
CTCGTAT9850.035.17712842
ACCGCAT10450.035.09515430
CATCTCG9900.034.99946239
CGTATGC10150.034.5807544
TCGTATG10150.034.5807543
GAGACCG10600.034.38626527
AGATCGG5150.033.20337317
CGAGACC11350.032.31227526
TGGCATC11200.031.33880436
TCGGAAG5400.030.8328620
GCATGGC12000.030.74952933
GATCGGA5500.030.68134918
GGCATCT11300.030.66324637
Produced by FastQC (version 0.11.5)