ERR1378938.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1378938.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625087
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAATAAATCTCGTATGCC	13491	2.1582595702678185	Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC	8232	1.316936682413808	Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	1762	0.28188076219790204	Illumina Paired End PCR Primer 2 (96% over 30bp)
CAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAA	670	0.1071850798368867	Illumina Paired End PCR Primer 2 (97% over 37bp)
GGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAATAAATCTCGTATGCCG	669	0.10702510210578688	Illumina Paired End PCR Primer 2 (96% over 32bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAATAAATCTCGTATGCCGTC	635	0.10158585924839261	Illumina Paired End PCR Primer 2 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	1985	36.72446	32
GTATGCC	1890	36.66569	45
GACCGGA	1985	36.611115	29
GAGACCG	1990	36.519123	27
CCGGAAT	2000	36.449028	31
AGACCGG	1995	36.4276	28
ACCGGAA	2010	36.15575	30
TCTCGTA	1930	36.13893	41
ATCTCGT	1945	35.86023	40
CTCGTAT	1980	35.339966	42
AGATCGG	1280	35.33109	17
CGTATGC	1985	35.137604	44
AATCTCG	2000	34.87407	39
TCGTATG	2000	34.87407	43
CCGAGAT	1300	34.787533	14
CGAGACC	2115	34.786304	26
CGAGATC	1310	34.69373	15
GATCGGA	1330	33.833683	18
GAGATCG	1340	33.7491	16
ATCGGAA	1335	33.706966	19