FastQCFastQC Report
Mon 6 Jun 2016
ERR1378937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707900
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTTAGATCTCGTATGCC113771.60714790224608Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC57950.8186184489334651Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11850.16739652493290014Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA13500.037.1662632
TCTCGTA13300.036.8792941
CTCGTAT13350.036.74117342
ATCTCGT13300.036.7101240
CGTATGC13450.036.63528444
GAGACCG13850.036.38949227
GTATGCC13400.036.2682645
CCGCCTT13900.036.25859531
AGACCGC13850.036.2270428
GACCGCC13900.036.09672529
TCGTATG13600.036.0657843
ACCGCCT14200.035.33411830
GCCTTAG14350.034.96477533
TTAGATC14350.034.8079836
TAGATCT14250.034.73645837
CCTTAGA14600.034.36606234
CTTAGAT14750.034.3216635
AGATCTC14350.034.1808138
GATCTCG14350.034.1808139
GTTCACG352.819472E-432.14250613
Produced by FastQC (version 0.11.5)