FastQCFastQC Report
Mon 6 Jun 2016
ERR1378931.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378931.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517602
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATATCTCGTATGCC88630.5840134633454621Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC57300.3775693495396026Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG12200.036.33130339
CGGCCAA12450.036.14391332
CTCGTAT12400.035.7464942
TCTCGTA12400.035.74531641
ATCTCGT12450.035.60175740
GACCGGC12700.035.2552629
GAGACCG12650.035.21674727
TCGTATG12650.035.0400443
CGTATGC12750.034.9416944
AGACCGG12950.034.74839828
CCGGCCA13050.034.4821331
ACCGGCC13100.034.35051730
GTATGCC13200.033.23912445
AGATCGG8250.031.0903417
GGCCAAT14700.030.61168533
GCCAATA14850.030.45398734
CGAGACC15400.029.36634626
CAATATC15400.029.36634636
GATCGGA8750.029.31375118
ATCGGAA9000.028.49947719
Produced by FastQC (version 0.11.5)