FastQCFastQC Report
Mon 6 Jun 2016
ERR1378930.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378930.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834456
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCGAAATCTCGTATGCC112140.6112983903674987Illumina Paired End PCR Primer 2 (97% over 36bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC67720.36915576061786165Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG16850.036.18622231
CTCGTAT16400.035.80916242
CGTTCGA17050.035.76174532
GTTCGAA17150.035.68441833
TCTCGTA16500.035.590241
ATCTCGT16700.035.16396740
TCGAAAT17400.035.04240435
TCGTATG16800.034.95656243
GACCGTT17450.034.81305729
ACCGTTC17600.034.51635430
CGTATGC17150.034.37436344
TTCGAAA17950.033.9686834
GAGACCG17900.033.9378727
AGACCGT17900.033.9378728
GTATGCC17800.033.2455345
CGAAATC18300.033.19605636
AATCTCG17950.032.71522539
AGATCGG8750.032.3993517
GATCGGA9350.030.56088618
ATCGGAA9400.030.39832919
Produced by FastQC (version 0.11.5)