FastQCFastQC Report
Mon 6 Jun 2016
ERR1378923.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378923.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020797
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTGATATCTCGTATGCC88240.864422603122854Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC51090.5004912827917793Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10810.1058976466427703Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG12100.037.74674239
CCGACTG12300.037.49881731
CGACTGA12350.037.34732
CTCGTAT12300.037.3195542
CGTATGC12500.036.7224444
ATCTCGT12500.036.7188440
GACCGAC12600.036.60598829
ACCGACT12600.036.60598830
TCTCGTA12650.036.461341
TCGTATG12600.036.43099243
GTATGCC12800.036.03754845
AGACCGA12900.035.75468428
GAGACCG13200.035.1125327
GACTGAT13800.033.58589633
AGATCGG8200.032.92579317
ACTGATA15150.030.89011434
TGATATC15050.030.79637136
GATCGGA8850.030.76174218
TCGGAAG8850.030.76174220
CGAGACC15450.030.29030626
Produced by FastQC (version 0.11.5)