FastQCFastQC Report
Mon 6 Jun 2016
ERR1378921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820415
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAACATATCTCGTATGCC120601.4699877501020826Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC54070.6590566969155853Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13140.1601628444141075Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG14550.036.03009839
TCTCGTA14550.035.8754641
CTCGTAT14800.035.42148242
GTATGCC14850.035.3043745
CGAAACA15350.035.1783432
CCGAAAC15300.034.99919531
AGACCGA15400.034.91802628
ATCTCGT14950.034.91558540
CGTATGC15100.034.86887444
TCGTATG15150.034.7537943
GACCGAA15500.034.6927529
ACCGAAA15600.034.32613430
GAGACCG15700.034.250827
CGAGACC16800.032.27604326
ATATCTC16900.031.41939538
ACATATC17000.031.36692636
CATATCT17100.031.05191437
AGATCGG7850.030.38146617
ATCGGAA8150.029.2631319
GATCGGA8250.029.18114718