FastQCFastQC Report
Mon 6 Jun 2016
ERR1378915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047050
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGCGAATCTCGTATGCC88380.8440857647676806Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC50150.4789647103767728Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12300.037.68416644
GCGAATC12250.037.65250436
CGAAGCG12300.037.4994432
TCTCGTA12400.037.1970341
ATCTCGT12600.036.96373740
CCGAAGC12550.036.9317231
CTCGTAT12550.036.7524442
CGAATCT12500.036.71945637
AAGCGAA12750.036.352434
AGCGAAT12750.036.352435
TCGTATG12700.036.32009543
GTATGCC13000.036.1742745
GACCGAA12900.036.10411529
GAAGCGA12800.036.03462233
ACCGAAG12900.035.929730
AGACCGA12950.035.79097428
AATCTCG13050.035.51671639
GAGACCG13150.035.24662427
AGATCGG6800.034.08037617
TCGGAAG7150.032.72678820
Produced by FastQC (version 0.11.5)