FastQCFastQC Report
Mon 6 Jun 2016
ERR1378914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280449
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGTAATCTCGTATGCC79640.621969324822777Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC42820.3344139438587558Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC9300.035.32469644
CTCGTAT9250.035.27238542
AGATCGG6000.035.24935517
CCGCTTG9600.035.1556131
ACCGCTT9550.034.86847730
TCTCGTA9350.034.6517741
TCGTATG9500.034.5810243
CGCTTGT9700.034.56122632
ATCTCGT9400.034.46745740
GACCGCT9700.034.32927329
AGACCGC9750.034.15322528
TCGGAAG6550.033.32000420
AATCTCG9800.033.0606239
GTATGCC10150.032.3664745
GAGACCG10350.032.1733327
GATCGGA6650.032.14227318
ATCGGAA6850.031.53227219
GTAATCT11000.030.06763537
GCTTGTA11250.029.99945433
GAGATCG7250.029.17188516
Produced by FastQC (version 0.11.5)