FastQCFastQC Report
Mon 6 Jun 2016
ERR1378913.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378913.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657130
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAATCTCGTATGCC112090.6764104204256758Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC78420.47322780952610843Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAC14050.036.99196631
CGTGACC14300.036.34525332
CTCGTAT13900.036.09731742
TCTCGTA13950.035.96793441
CGTATGC14050.035.71085744
ATCTCGT14250.035.21071240
TCGTATG14350.034.96428743
AGACCGT15150.034.3060828
ACCGTGA15150.034.15756630
GTATGCC14750.033.55848745
GACCGTG15450.033.49431629
GAGACCG15600.033.4607127
AATCTCG15300.032.79429239
GACCAAT16200.031.94365135
ACCAATC16600.030.90378236
GTGACCA16850.030.84493333
CCAATCT17100.029.86858237
AGATCGG12200.029.32304417
CGAGACC18150.028.88358126
GATCGGA12550.028.50526818
Produced by FastQC (version 0.11.5)