FastQCFastQC Report
Mon 6 Jun 2016
ERR1378903.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378903.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968020
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCC98201.0144418503749923Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC52190.5391417532695606Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11140.11508026693663354Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13050.036.8976142
TCTCGTA13150.036.6170241
ATCTCGT13150.036.4459140
TATCTCG13150.036.4459139
TCGTATG13300.036.37134643
CGTATGC13400.036.26782244
GTATGCC13600.035.73447445
CCGTGCC13800.035.37962331
CGTGCCA14100.034.94600732
GACCGTG14000.034.7134929
GAGACCG14150.034.66351327
ACCGTGC14150.034.5045130
AGACCGT14200.034.38301528
GCCATAT15150.033.26656335
CGAGACC15350.031.95366326
CCATATC15300.031.91268236
TGCCATA15750.031.71355834
GTGCCAT15950.031.31589533
CATATCT15900.030.14237437
ATATCTC16050.030.0008638
Produced by FastQC (version 0.11.5)