FastQCFastQC Report
Mon 6 Jun 2016
ERR1378902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166568
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACAGCATCTCGTATGCC105160.9014476652882644Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC58840.5043855137463054Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13430.11512402191728213Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAG13600.037.22358732
TCTCGTA13400.036.60538541
CCGTACA13850.036.5516831
CTCGTAT13450.036.46930742
CATCTCG13650.035.93495639
CGTATGC13850.035.57697344
TCGTATG13750.035.5084543
ATCTCGT13850.035.4160440
GACCGTA14300.035.40145529
ACCGTAC14350.035.27810730
GTATGCC14000.035.0350845
AGACCGT14550.034.79318228
GAGACCG14750.034.6264927
GTACAGC14950.034.01276433
AGATCGG8850.030.76215417
GCATCTC15950.030.7531138
CGAGACC16750.030.22333526
TACAGCA16900.030.08821334
ATCGGAA9050.030.08232919
GATCGGA9250.029.67513818
Produced by FastQC (version 0.11.5)