FastQCFastQC Report
Mon 6 Jun 2016
ERR1378896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233927
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAATGATCTCGTATGCC79530.6445275936096706Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC42350.34321317225411224Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA10300.034.95073341
CGTATGC10400.034.6146744
CTCGTAT10450.034.4490542
CCGTGAA10700.034.2749931
ATCTCGT10550.034.12251740
CGTGAAT10750.034.1155732
TCGTATG10650.033.80211643
GTATGCC10850.033.38640245
ACCGTGA11150.033.09348730
AGACCGT11250.032.7993228
GACCGTG11350.032.31210729
GAGACCG11800.031.46121227
AGATCGG7100.029.47122217
GATCTCG12700.028.3458739
CGAGACC13200.027.95396826
GATCGGA7700.027.46696518
AATGATC13350.027.30280536
ATCGGAA7750.026.9994419
TCGGAAG8000.026.15570820
CGAGATC8100.026.11056915
Produced by FastQC (version 0.11.5)