FastQCFastQC Report
Mon 6 Jun 2016
ERR1378895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333626
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTATGCC86760.6505572026940086Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC46230.34664891056413116Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG11450.035.17364533
ATCGATC11500.034.62942536
CCGTAAT11650.034.3766831
TAATCGA11650.034.3766834
CGTATGC11750.034.27816844
TCTCGTA11600.034.13693641
CGTAATC11850.033.9863532
ACCGTAA11800.033.9396930
CTCGTAT11850.033.7990242
AATCGAT11800.033.74901635
GACCGTA11900.033.6544829
GAGACCG12150.033.14718227
ATCTCGT12050.033.04883240
AGACCGT12150.032.96228
TCGTATG12250.032.8790643
GTATGCC12350.032.43063445
TCGATCT13200.030.51047337
AGATCGG7500.030.29911817
CGAGCTT300.005148284629.99912620
CGAGACC14150.028.46206926
Produced by FastQC (version 0.11.5)