FastQCFastQC Report
Mon 6 Jun 2016
ERR1378889.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378889.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853749
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35020.41019081720739936Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGACATATCTCGTATGCCGTC22280.26096663070762016Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGACATATCTCGTATGCC17650.20673523482897196Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2300.039.12702643
TACGGAG250.002108313435.9947527
ATGCCGT2650.033.959344
TGCCGTC2750.032.7244245
AGATCGG4400.031.69992425
GATCGGA4450.031.34374426
TCGGAAG4800.030.4643128
ATCGGAA4850.028.75869627
CCGAGAT5200.026.82301122
AATGCCG9350.025.74491118
ATGCCGA9500.025.57521819
TGCCGAG9750.024.91944320
GAGATCG5800.024.04821824
CGAGATC5950.023.44196123
GCCGAGA9900.023.4056821
GAATGCC10650.022.6023417
TCTCGTA4300.021.45036138
CGTATGC4350.021.20504841
CTCGTAT4350.021.20504839
TCGTATG4350.021.20504840
Produced by FastQC (version 0.11.5)