FastQCFastQC Report
Mon 6 Jun 2016
ERR1378888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903537
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT28210.31221742994476154Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGTAACATCTCGTATGCCGTC17890.19799963919573854Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGTAACATCTCGTATGCC17440.19301921227354277Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1850.031.61512843
GATCGGA4550.028.18102526
ATCGGAA4600.027.87470827
TCGGAAG4900.027.5453628
AGATCGG4600.027.38719625
TGCCGTC2150.027.20371445
ATGCCGT2300.026.4076244
ATGCCGA8250.023.99639919
AATGCCG8450.023.6933618
TGCCGAG8500.023.02595520
CCGAGAT5550.022.69929722
CGAGATC5850.021.53522923
CGGAAGA6500.021.4571329
GAGATCG6000.020.9968524
TCGTATG3250.020.76496543
GCCGAGA9250.020.42936521
GAATGCC10050.020.3689517
CTCGTAT3300.019.76866742
CGTATGC3350.019.47361244
AGCGGTT10650.019.2480344
Produced by FastQC (version 0.11.5)