FastQCFastQC Report
Mon 6 Jun 2016
ERR1378887.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378887.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899126
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT37320.41506974550841597Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCCGTC22290.247907412309287Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCC21830.24279133291663238Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1950.038.073343
AGATCGG6000.033.3681125
ATCGGAA6300.031.77915227
GATCGGA6400.031.28260626
TCGGAAG6650.031.12139328
ATGCCGT2350.030.63538244
CCGAGAT7200.027.8067622
GAGATCG7550.026.21975324
TGCCGTC2750.026.17932745
CGAGATC7700.025.70897923
AATGCCG11350.024.3782318
TGCCGAG11750.023.73978220
TCTCGTA4550.023.73400541
ATGCCGA11750.023.54833219
CTCGTAT4600.023.47602542
CGTATGC4650.023.22359744
TCGTATG4650.023.22359743
CGGAAGA9050.022.8694829
ACCGGCT4650.022.2547130
GAATGCC12650.022.22861517
Produced by FastQC (version 0.11.5)