FastQCFastQC Report
Mon 6 Jun 2016
ERR1378886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034344
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT20920.2022537956424555Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGAAGATCTCGTATGCC14460.13979875167255768Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGAAGATCTCGTATGCCGTC13880.13419133286411486Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1850.031.61869843
AGATCGG3600.028.74456425
GATCGGA3500.028.28036526
ATCGGAA3650.027.73448227
TCGGAAG4150.026.01917528
TGCCGTC2250.025.99759945
ATGCCGT2300.025.43243244
CTCGTAT3600.022.49792142
AATGCCG7750.021.77007518
CGTATGC3650.021.5733544
TCGTATG3650.021.5733543
ATGCCGA7850.021.4927519
TGCCGAG7900.020.50245320
TCTCGTA3850.020.45265641
AGACCGC4150.020.05644828
CGCTGAA4050.019.99815432
ATCTCGT4000.019.6856840
CGAGATC5300.019.1001623
CCGAGAT5200.019.0348622
CGGTGAA951.31777E-518.94378725
Produced by FastQC (version 0.11.5)