FastQCFastQC Report
Mon 6 Jun 2016
ERR1378884.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378884.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767988
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27870.36289629525461337Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCAAGATCTCGTATGCCGTC20250.2636759949374209Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCAAGATCTCGTATGCC19930.25950926316557027Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2400.038.43249545
TATGCCG2650.035.6557343
ATGCCGT2700.034.16221644
ATCGGAA4950.033.63198527
TCGGAAG5250.032.995728
GATCGGA5100.032.64280726
AGATCGG5200.032.0150625
CCGAGAT5850.029.22696522
TGCCGAG9750.027.45796420
TGAACCG507.161704E-526.99648510
ATGCCGA10050.026.6383219
AATGCCG10050.026.4144718
GAGATCG6400.026.01223824
CGAGATC6600.025.22398823
GCCGAGA10600.024.61943421
CGGAAGA7150.024.22761529
GAATGCC11250.023.796917
GGAATGC12400.021.5899316
CTCGTAT4300.021.45069539
CGTATGC4350.021.20413441
Produced by FastQC (version 0.11.5)