FastQCFastQC Report
Mon 6 Jun 2016
ERR1378883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004536
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT29400.2926724378220392Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACAACATCTCGTATGCC26450.2633056455915965Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACAACATCTCGTATGCCGTC19600.19511495854802616Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA5150.033.1971627
GATCGGA5250.032.56483526
TATGCCG2650.032.26083443
AGATCGG5250.032.1363525
TCGGAAG5450.031.78255728
ATGCCGT3050.028.02990344
CTCGTAT5750.027.77975342
TCTCGTA5750.027.77975341
GAGATCG6100.027.65833524
TGCCGTC3100.027.57780645
ATCTCGT6050.026.77410540
CGTATGC6050.026.40224344
ACCGGAC6050.026.39961230
GACCGGA6050.026.39961229
CGGACAA6100.026.18974132
CGAGATC6550.026.10158523
GTATGCC6300.026.06874845
CCGGACA6150.025.97681831
CCGAGAT6600.025.90384522
AGACCGG6300.025.70908428
Produced by FastQC (version 0.11.5)