FastQCFastQC Report
Mon 6 Jun 2016
ERR1378882.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378882.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726377
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT34180.4705545467436331Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCCGTC26230.3611072487151989Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCC21210.2919971309664265Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3250.039.458743
ATGCCGT3700.035.26773544
ATCGGAA4550.032.13612427
TCGGAAG4600.031.78681628
TGCCGTC4050.031.6643945
GATCGGA4850.030.14832126
AGATCGG5000.029.24588225
AATGCCG10300.026.86330218
CCGAGAT5700.025.25960222
CGAGATC5700.025.25960223
GAGATCG5700.025.25960224
GTCGTTA5300.025.04192230
CGTATGC5200.024.66168841
TCTCGTA5200.024.6582938
CGTTAAT5250.024.42513332
TGCCGAG11350.024.37982720
GAATGCC11450.024.16524117
TCGTTAA5400.024.16326531
CGTCGTT5500.024.13130629
TCGTATG5350.023.9702440
Produced by FastQC (version 0.11.5)