FastQCFastQC Report
Mon 6 Jun 2016
ERR1378879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855507
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT39350.4599611692247989Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCCGTC23560.2753922527810994Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCC16040.18749116021259907Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.036.50782443
TGCCGTC2950.033.5578445
ATGCCGT3000.032.24857344
ATCGGAA6300.031.77873827
TCGGAAG6500.031.49676928
GATCGGA6450.031.38845826
AGATCGG6550.030.90924525
GAGATCG7450.027.4771924
CGAGATC7400.027.35885623
CCGAGAT7800.026.24423822
AATGCCG11700.026.14810818
ATGCCGA11700.026.14810819
TGCCGAG12250.024.9741120
GCCGAGA13050.023.61550521
GAATGCC13150.023.26485317
CGGAAGA8900.023.00325629
TCTCGTA4500.021.49528338
CTCGTAT4600.021.03168139
GGAATGC14800.020.97512416
CGGTTGT4850.020.87414729
Produced by FastQC (version 0.11.5)