FastQCFastQC Report
Mon 6 Jun 2016
ERR1378878.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378878.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074032
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35510.33062329614015223Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGTTATCTCGTATGCCGTC22400.20855989393239682Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGTTATCTCGTATGCC18570.17289987635377715Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.040.75147243
ATGCCGT3200.033.7473144
TGCCGTC3500.030.85468545
GATCGGA4950.029.99481826
TCGGAAG5200.029.8547828
AGATCGG5050.029.4008625
ATCGGAA5150.028.3931527
ATGCCGA10400.025.95705219
AATGCCG10550.025.37476218
TGCCGAG10750.025.32120520
CCGAGAT6450.023.71683322
CTCGTAT4650.023.22395539
CGTATGC4750.022.73503141
AGACCGT4750.022.73291425
GAATGCC12000.022.6835817
GCCGAGA11450.022.59434921
CGAGATC7000.021.85336723
TCTCGTA5050.021.82791138
TATCTCG5100.021.17478236
TCGTATG5100.021.17478240
Produced by FastQC (version 0.11.5)