FastQCFastQC Report
Mon 6 Jun 2016
ERR1378877.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378877.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117761
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT30320.2712565566342Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCCGTC19340.17302446587418957Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCC19180.17159303285765024Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2750.037.63295743
ATGCCGT3200.032.34082444
GATCGGA3450.030.64528726
TGCCGTC3500.029.56875445
ATCGGAA3600.029.368427
TCGGAAG3900.028.2654128
AGATCGG3850.027.4613625
AATGCCG9150.023.35540618
ATGCCGA9400.022.73425319
TGCCGAG9500.022.73173320
GAATGCC10550.020.68255417
GAGATCG5200.020.33196824
CTCGTAT5150.020.0952739
GACCGTT5300.019.95013229
CGTATGC5200.019.90204441
TCGTATG5200.019.90204440
AGACCGT5350.019.76368128
GAGCGGT11250.019.6175373
TCTCGTA5300.019.52653541
GCGGTTC11350.019.4429535
Produced by FastQC (version 0.11.5)